Anticorps Recombinant de lapin anti-Ins1

Ins1 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240798G4

N° de cat : 83867-1-PBS

Synonymes

Insulin, insulin1, insulin I, Ins2 rs1, Ins-1

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Galerie de données de validation

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  WB analysis of rat pancreas using 83867-1-RR (same clone as 83867-1-PBS)

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 83867-1-RR (insulin1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83867-1-RR (Ins1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using insulin1 antibody (83867-1-RR, Clone: 240798G4, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).SST-Specific antibody (84351-1-RR, Clone: 240816D9,red ) at dilution of 1:400 and FlexAble CoraLite® Plus 594 Antibody Labeling Kit for Rabbit IgG(KFA009), Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 83867-1-RR (same clone as 83867-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Ins1 antibody (83867-1-RR, Clone: 240798G4 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83867-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83867-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83867-1-RR against Mouse Ins1 were performed. The affinity constant is 0.21 nM.

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Informations sur le produit

83867-1-PBS cible Ins1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Ins1 Protéine recombinante Ag29988
• Nom complet: insulin I
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: NM_008386
• Symbole du gène: Ins1
• Identification du gène (NCBI): 16333
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Insulin 1 is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

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