Anticorps Monoclonal anti-INS

INS Monoclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications(54)

CloneNo.

4B6A7

Avis (1)

N° de cat : 66198-1-Ig

Synonymes

insulin, 4B6A7

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Galerie de données de validation

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  IHC staining of human pancreas using 66198-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:5000 (under 10x lens).

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  IHC staining of human pancreas using 66198-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:5000 (under 40x lens).

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  IHC staining of rat pancreas using 66198-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 66198-1-Ig (Insulin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 66198-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 66198-1-Ig (Insulin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 66198-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of mouse pancreas using 66198-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66198-1-Ig (Insulin Antibody) at dilution of 1:1000 (under 40x lens).

• 

  IF Staining of human pancreas using 66198-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 66198-1-Ig (Insulin antibody) at dilution of 1:100 and CoraLite594-Conjugated Goat Anti-Mouse IgG(H+L). The α cells of pancreas was labelled in green with 15954-1-AP (Glucagon antibody).

• 

  IF Staining of mouse pancreas using 66198-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using INS antibody (66198-1-Ig, Clone: 4B6A7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), Somatostatin antibody (24496-1-AP, green).

• 

  IF Staining of mouse pancreas using 66198-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using INS antibody (66198-1-Ig, Clone: 4B6A7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 66198-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using INS antibody (66198-1-Ig, Clone: 4B6A7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 66198-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 66198-1-Ig (Insulin antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu pancréatique humain, tissu pancréatique de rat, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu pancréatique humain, tissu pancréatique de souris

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:1000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 10 publications below
• IF: See 44 publications below

Informations sur le produit

66198-1-Ig cible INS dans les applications de WB, IHC, IF-P, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: INS Protéine recombinante Ag8630
• Nom complet: INS
• Masse moléculaire calculée: 110 aa, 12 kDa
• Numéro d’acquisition GenBank: BC005255
• Symbole du gène: INS
• Identification du gène (NCBI): 3630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, pro-INS, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus. INS may also exist 36 kDa form corresponding to the hexameric INS form.

Publications

Species	Application	Title
mouse	IF	Front Immunol; Metabolic effects of CCL5 deficiency in lean and obese mice; Authors - Hui Zhou
mouse	IF	Food Chem X; Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.; Authors - Jingyu Fang
mouse	IF	Clin Sci (Lond); Mitochondrial protein MPV17 promotes β-cell apoptosis in diabetogenesis; Authors - Qiaoli Tang
mouse	IHC,IF	Int Immunopharmacol; RAGE deficiency obstructs high uric acid-induced oxidative stress and inflammatory response; Authors - Hairong Zhao
mouse	IF	FASEB J; cGAS-STING mediates cytoplasmic mitochondrial-DNA-induced inflammatory signal transduction during accelerated senescence of pancreatic β-cells induced by metabolic stress.; Authors - Huiqing Hu
mouse	IF	Ecotoxicol Environ Saf; Green light exposure aggravates high-fat diet feeding-induced hepatic steatosis and pancreatic dysfunction in male mice.; Authors - Shiyao Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vincent (Verified Customer) (07-08-2019)

This antibody offers a good detection of Insulin expression in both DM mice and WT mice. The insulin expression can be observed in the islet cells of pancreatic tissue.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: pancreatic tissue/ islet cells

INS Antibody Immunofluorescence, validation (1:500 dilution) in pancreatic tissue/ islet cells (Cat no:66198-1-Ig)

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