Anticorps Monoclonal anti-Integrin Alpha 3

• Phare
• Validé par KD/KO

Integrin Alpha 3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F9G4

N° de cat : 66070-1-PBS

Synonymes

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

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Galerie de données de validation

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  WB analysis of A549 using 66070-1-Ig (same clone as 66070-1-PBS)

  WB result of Integrin Alpha 3 antibody (66070-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 3 transfected A549 cells. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66070-1-Ig (same clone as 66070-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (ITGA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66070-1-Ig (same clone as 66070-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66070-1-Ig (same clone as 66070-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66070-1-Ig (same clone as 66070-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66070-1-Ig (same clone as 66070-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66070-1-Ig (same clone as 66070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66070-1-Ig (same clone as 66070-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66070-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66070-1-PBS cible Integrin Alpha 3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Integrin Alpha 3 Protéine recombinante Ag17293
• Nom complet: integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• Masse moléculaire calculée: 1066 aa, 119 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC150190
• Symbole du gène: Integrin alpha 3
• Identification du gène (NCBI): 3675
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

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