Anticorps Recombinant de lapin anti-Integrin Alpha V

Integrin Alpha V Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242376D3

N° de cat : 84883-5-PBS

Synonymes

ITGAV, 242376D3, CD51, Integrin alpha-V, Integrin alpha-V heavy chain

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Galerie de données de validation

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  WB analysis using 84883-5-RR (same clone as 84883-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocellular cancer using 84883-5-RR (same clone as 84883-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocellular cancer using 84883-5-RR (same clone as 84883-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84883-5-RR (same clone as 84883-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84883-5-RR (same clone as 84883-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 84883-5-RR (same clone as 84883-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (84883-5-RR, Clone: 242376D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84883-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84883-5-RR against Human Integrin Alpha V were performed. The affinity constant is 0.121 nM.

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Informations sur le produit

84883-5-PBS cible Integrin Alpha V dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Integrin Alpha V Protéine recombinante Ag25825
• Nom complet: integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• Masse moléculaire calculée: 1048 aa, 116 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC136442
• Symbole du gène: Integrin alpha V
• Identification du gène (NCBI): 3685
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha V, also known as CD51, is a type I transmembrane glycoprotein composed of an extracellular domain, a transmembrane region and cytoplasmic domain (PMID: 2430295; 1690718). It can form heterodimers with one of the five beta integrin subunits (beta 1, 3, 5, 6, 8) that recognize ligands containing the sequence Arg-Gly-Asp, such as vitronectin, fibronectin, and osteopontin (PMID: 37087799). Integrin alpha V mainly plays a role in extracellular matrix-mediated cell adhesion and migration, differentiation, wound healing, inflammation, tumorigenesis, proliferation, angiogenesis, and metastasis.

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