Anticorps Polyclonal de lapin anti-Integrin Beta 1/CD29

• Phare
• Validé par KD/KO

Integrin Beta 1/CD29 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 12594-1-PBS

Synonymes

ITGB1, CD29, FNRB, Glycoprotein IIa, GPIIA

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Galerie de données de validation

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  WB analysis using 12594-1-AP (same clone as 12594-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  WB analysis using 12594-1-AP (same clone as 12594-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12594-1-AP (same clone as 12594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 12594-1-AP (same clone as 12594-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12594-1-AP (same clone as 12594-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12594-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12594-1-PBS cible Integrin Beta 1/CD29 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin Beta 1/CD29 Protéine recombinante Ag3078
• Nom complet: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 100-130 kDa
• Numéro d’acquisition GenBank: BC020057
• Symbole du gène: Integrin beta 1
• Identification du gène (NCBI): 3688
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

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