Anticorps Polyclonal de lapin anti-Integrin alpha 1

Integrin alpha 1 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 29042-1-AP

Synonymes

ITGA1, CD49 antigen-like family member A, CD49a, integrin, alpha 1, Laminin and collagen receptor

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Galerie de données de validation

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  WB analysis using 29042-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 29042-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29042-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 29042-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Integrin alpha-1 antibody (29042-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 29042-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using Integrin alpha-1 antibody (29042-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu placentaire humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu placentaire humain,
• Résultats positifs en IF-Fro: tissu splénique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

29042-1-AP cible Integrin alpha 1 dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin alpha 1 Protéine recombinante Ag30613
• Nom complet: integrin, alpha 1
• Masse moléculaire calculée: 1179 aa, 131 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC137121
• Symbole du gène: Integrin alpha 1
• Identification du gène (NCBI): 3672
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Publications

Species	Application	Title
human	WB	Development; TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration; Authors - Binbin Li
human	WB	PeerJ; Production of recombinant human epidermal growth factor fused with HaloTag protein and characterisation of its biological functions; Authors - Mengru Bai

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