Anticorps Polyclonal de lapin anti-Integrin alpha 2

Integrin alpha 2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 30703-1-AP

Synonymes

ITGA2, BR, CD49B, Collagen receptor, GPIa

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Galerie de données de validation

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  WB analysis using 30703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30703-1-AP (Integrin alpha 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30703-1-AP (Integrin alpha-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 30703-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ITGA2 antibody (30703-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules A431, cellules HeLa, cellules MDA-MB-231
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30703-1-AP cible Integrin alpha 2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin alpha 2 Protéine recombinante Ag32771
• Nom complet: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• Masse moléculaire calculée: 126 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: NM_002203
• Symbole du gène: Integrin alpha 2
• Identification du gène (NCBI): 3673
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-2 (ITGA2, CD49b) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor (VLA-2) for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin.

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