Anticorps Monoclonal anti-Integrin alpha-5/CD49e

• Phare
• Validé par KD/KO

Integrin alpha-5/CD49e Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B9E5

N° de cat : 68561-1-PBS

Synonymes

Integrin Alpha-5, ITGA5, 3B9E5, CD49 antigen-like family member E, CD49e

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Galerie de données de validation

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  WB analysis using 68561-1-Ig (same clone as 68561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68561-1-PBS in a different storage buffer formulation.

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  WB analysis of HAP1 using 68561-1-Ig (same clone as 68561-1-PBS)

  WB result of Integrin alpha-5/CD49e antibody (68561-1-Ig; 1:8000; room temperature for 1.5 hours) with wild-type and Integrin alpha-5/CD49e knockout HAP1 cells. This data was developed using the same antibody clone with 68561-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68561-1-Ig (same clone as 68561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68561-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 68561-1-Ig (same clone as 68561-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68561-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68561-1-PBS cible Integrin alpha-5/CD49e dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Integrin alpha-5/CD49e Protéine recombinante Ag33642
• Nom complet: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Masse moléculaire calculée: 115 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC008786
• Symbole du gène: Integrin alpha 5
• Identification du gène (NCBI): 3678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

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