Anticorps Recombinant de lapin anti-Integrin alpha-5/CD49e

• Phare
• Validé par KD/KO

Integrin alpha-5/CD49e Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241684E8

N° de cat : 84468-5-RR

Synonymes

Integrin Alpha-5, ITGA5, 241684E8, CD49 antigen-like family member E, CD49e

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Galerie de données de validation

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  WB analysis of HAP1 using 84468-5-RR

  WB result of Integrin alpha-5/CD49e antibody (84468-5-RR; 1:4000; room temperature for 1.5 hours) with wild-type and sg-Integrin alpha-5/CD49e transfected HAP1 cells.

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  WB analysis using 84468-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84468-5-RR (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84468-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84468-5-RR against Human Integrin alpha-5 were performed. The affinity constant is 1.41 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84468-5-RR cible Integrin alpha-5/CD49e dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Masse moléculaire calculée: 115 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC008786
• Symbole du gène: Integrin alpha 5
• Identification du gène (NCBI): 3678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

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