Anticorps Polyclonal de lapin anti-Integrin alpha-8

Integrin alpha-8 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30714-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 30714-1-AP (same clone as 30714-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30714-1-AP (Integrin alpha-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Integrin alpha-8 antibody (30714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 30714-1-AP (same clone as 30714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Integrin alpha-8 antibody (30714-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30714-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30714-1-PBS cible Integrin alpha-8 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin alpha-8 Protéine recombinante Ag33507
• Nom complet: integrin, alpha 8
• Masse moléculaire calculée: 117 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: NM_003638
• Symbole du gène: Integrin alpha 8
• Identification du gène (NCBI): 8516
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. Integrin alpha-8 is a single-pass type I membrane protein that forms a heterodimer with integrin beta-1 to serve as a receptor for RGD-containing matrix molecules (fibronectin, vitronectin, tenascin C, osteopontin, and nephronectin) (PMID: 33000355). Integrin alpha-8 beta-1 plays a critical role in epithelial-mesenchymal interactions and functions in the genesis of kidney and probably of other organs (PMID: 9054500; 19769957). The apparent molecular weight of integrin alpha-8 is larger than the calculated molecular weight of 117 kDa, possibly due to glycosylation (PMID: 7768999).

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