Anticorps Monoclonal anti-Integrin alpha-E

Integrin alpha-E Monoclonal Antibody for IF/ICC, FC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF/ICC, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

4B6G6

N° de cat : 68893-3-Ig

Synonymes

CD103, 4B6G6, Integrin alpha E, HUMINAE, HML-1 antigen

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Galerie de données de validation

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  IF Staining of human PBMCs using 68893-3-Ig

  Immunofluorescent analysis of un-fixed 5 µg/mL PHA ( 3 days) treated human PBMCs using Integrin alpha-E antibody (68893-3-Ig, Clone: 4B6G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005).

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  FC experiment of human PBMCs using 68893-3-Ig

  1x10^6 untreated human PBMCs (left) and 5 µg/mL PHA (3 days) treated human PBMCs (right) were surface stained with 0.2 μg Integrin Alpha-E Monoclonal Antibody (68893-3-Ig, Clone: 4B6G6) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: 5 µg/mL PHA ( 3 days) treated human PBMCs,
• Résultats positifs en cytométrie: 5 µg/mL PHA for 3 days human PBMCs,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68893-3-Ig cible Integrin alpha-E dans les applications de IF/ICC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Integrin alpha-E Protéine recombinante Ag27410
• Nom complet: integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
• Masse moléculaire calculée: 1179 aa, 130 kDa
• Numéro d’acquisition GenBank: BC113436
• Symbole du gène: ITGAE
• Identification du gène (NCBI): 3682
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrin alpha-E (CD103) is a member of the integrin family, specifically the αEβ7 heterodimer. It plays a crucial role in the immune system, particularly in the interaction between leukocytes and epithelial cells. Integrin alpha-E (CD103) is a critical component of the immune system, particularly in the context of mucosal immunity. Its ability to bind to E-cadherin and regulate the production of IFN-I in cDCs during viral infections highlights its multifaceted role in immune regulation and disease prevention. (PMID: 33584680)

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