Anticorps Recombinant de lapin anti-Involucrin

Involucrin Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240566D8

N° de cat : 83649-5-PBS

Synonymes

IVL, 240566D8

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Galerie de données de validation

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  WB analysis using 83649-5-RR (same clone as 83649-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83649-5-RR (Involucrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83649-5-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 83649-5-RR (same clone as 83649-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83649-5-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 83649-5-RR (same clone as 83649-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83649-5-RR (Involucrin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83649-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83649-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83649-5-RR against Human Involucrin were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83649-5-PBS cible Involucrin dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Involucrin Protéine recombinante Ag29585
• Nom complet: involucrin
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: NM_005547
• Symbole du gène: Involucrin
• Identification du gène (NCBI): 3713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502, 12150517)

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