Anticorps Recombinant de lapin anti-Islet 1

Islet 1 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240833D7

N° de cat : 83784-4-PBS

Synonymes

ISL1, 240833D7, Insulin gene enhancer protein ISL-1, Isl 1, ISL LIM homeobox 1

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Galerie de données de validation

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  WB analysis using 83784-4-RR (same clone as 83784-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83784-4-RR (Islet 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83784-4-RR (same clone as 83784-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83784-4-RR (Islet 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83784-4-RR (same clone as 83784-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83784-4-RR (Islet 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 83784-4-RR (same clone as 83784-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Islet 1 antibody (83784-4-RR, Clone: 240833D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83784-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83784-4-RR against Human Islet 1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83784-4-PBS cible Islet 1 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Islet 1 Protéine recombinante Ag33541
• Nom complet: ISL LIM homeobox 1
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39-45 kDa
• Numéro d’acquisition GenBank: BC031213
• Symbole du gène: Islet 1
• Identification du gène (NCBI): 3670
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.

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