Anticorps Recombinant de lapin anti-Islet 1

Islet 1 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240833B2

N° de cat : 83784-6-PBS

Synonymes

ISL1, ISLET1, ISL LIM homeobox 1, Isl 1, Insulin gene enhancer protein ISL-1

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Galerie de données de validation

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  WB analysis using 83784-6-RR (same clone as 83784-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83784-6-RR (Islet 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83784-6-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00771-4

  Sandwich ELISA standard curve of MP00771-4, Human Islet 1 Recombinant Matched Antibody Pair - PBS only. 83784-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33541. 83784-6-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP00771-4

  The mean Islet 1 concentration was determined to be 1.05 ng/mL in SH-SY5Y cell extract based on a 3.40 mg/mL extract load.

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Informations sur le produit

83784-6-PBS cible Islet 1 dans les applications de WB, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Islet 1 Protéine recombinante Ag33541
• Nom complet: ISL LIM homeobox 1
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39-45 kDa
• Numéro d’acquisition GenBank: BC031213
• Symbole du gène: Islet 1
• Identification du gène (NCBI): 3670
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.

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