Anticorps Monoclonal anti-JAK1

• Phare
• Validé par KD/KO

JAK1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

31

CloneNo.

3H7A8

Avis (1)

N° de cat : 66466-1-Ig

Synonymes

JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1

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Galerie de données de validation

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  WB analysis using 66466-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis using 66466-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 66466-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66466-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66466-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66466-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66466-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules Daudi, cellules Jurkat, cellules NIH/3T3, cellules PC-12, cellules Ramos, cellules RAW 264.7, tissu splénique de rat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 29 publications below
• IF: See 1 publications below

Informations sur le produit

66466-1-Ig cible JAK1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: JAK1 Protéine recombinante Ag17940
• Nom complet: Janus kinase 1 (a protein tyrosine kinase)
• Masse moléculaire calculée: 1154 aa, 133 kDa
• Poids moléculaire observé: 133 kDa
• Numéro d’acquisition GenBank: BC132729
• Symbole du gène: JAK1
• Identification du gène (NCBI): 3716
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.

Publications

Species	Application	Title
human	IF	Adv Sci (Weinh); EHBP1L1 Drives Immune Evasion in Renal Cell Carcinoma through Binding and Stabilizing JAK1; Authors - Yihui Pan
mouse	WB	Cell Rep; Macrophage autophagy deficiency-induced CEBPB accumulation alleviates atopic dermatitis via impairing M2 polarization; Authors - Yongcheng Zhu
human,mouse	WB	Cell Chem Biol; Small-molecule MHC-II inducers promote immune detection and anti-cancer immunity via editing cancer metabolism; Authors - Ling Huang
mouse	WB	Antioxidants (Basel); Fibroblast Upregulation of Vitamin D Receptor Represents a Self-Protective Response to Limit Fibroblast Proliferation and Activation during Pulmonary Fibrosis; Authors - Juan Wei
mouse	WB	Antioxidants (Basel); Periplaneta americana Extract Pretreatment Alleviates Oxidative Stress and Inflammation and Increases the Abundance of Gut Akkermansia muciniphila in Diquat-Induced Mice; Authors - Shiyi Lu
mouse	WB	Oxid Med Cell Longev; Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response.; Authors - Guanghui Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christine (Verified Customer) (07-12-2022)

Signal is at the expected molecular weight, very low aspecific staining on the membrane, signal is proportional to the lysate quantity loaded. Need quite a large quantity of lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE1 cells and Huh7 cells (human)

JAK1 Antibody Western Blot validation (1:1000 dilution) in RPE1 cells and Huh7 cells (human) (Cat no:66466-1-Ig)