Anticorps Monoclonal anti-JAK1

• Phare
• Validé par KD/KO

JAK1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

3H7A8

N° de cat : 66466-1-PBS

Synonymes

JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1

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Galerie de données de validation

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  WB analysis using 66466-1-Ig (same clone as 66466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

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  WB analysis using 66466-1-Ig (same clone as 66466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 66466-1-Ig (same clone as 66466-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66466-1-Ig (JAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66466-1-Ig (same clone as 66466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66466-1-Ig (JAK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66466-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66466-1-PBS cible JAK1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: JAK1 Protéine recombinante Ag17940
• Nom complet: Janus kinase 1 (a protein tyrosine kinase)
• Masse moléculaire calculée: 1154 aa, 133 kDa
• Poids moléculaire observé: 133 kDa
• Numéro d’acquisition GenBank: BC132729
• Symbole du gène: JAK1
• Identification du gène (NCBI): 3716
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.

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