Anticorps Recombinant de lapin anti-JAK3

JAK3 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

3N1

N° de cat : 80331-1-RR

Synonymes

3N1, EC:2.7.10.2, JAK 3, JAK-3, JAKL

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Galerie de données de validation

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  WB analysis using 80331-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80331-1-RR (JAK3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 80331-1-RR

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 80331-1-RR (JAK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 80331-1-RR

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 80331-1-RR (JAK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below

Informations sur le produit

80331-1-RR cible JAK3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Janus kinase 3 (a protein tyrosine kinase, leukocyte)
• Masse moléculaire calculée: 1124 aa, 125 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: BC028068
• Symbole du gène: JAK3
• Identification du gène (NCBI): 3718
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

JAK3 is a non-receptor tyrosine kinase, predominantly expressed in hematopoietic cells and that has been implicated in the signal transduction of the common gamma chain subfamily of cytokine receptors. As a result, JAK3 plays an essential role in hematopoieisis during T cell development. JAK3 belongs to the Janus kinase family of receptor-associated tyrosine kinases located in cytoplasm adjacent to the plasma membrane. (PMID: 19747563, PMID: 33767339)

Publications

Species	Application	Title
human	WB	Front Immunol; Construction of disulfidptosis-based immune response prediction model with artificial intelligence and validation of the pivotal grouping oncogene c-MET in regulating T cell exhaustion; Authors - Pengping Li
human	IHC	Gene; The JAK-STAT signaling-related signature serves as a prognostic and predictive biomarker for renal cell carcinoma immunotherapy; Authors - Szehoi Chan
mouse	IHC	Front Genet; Identification of apoptosis-immune-related gene signature and construction of diagnostic model for sepsis based on single-cell sequencing and bulk transcriptome analysis; Authors - Zhongyi Sun
human	IHC	Clin Exp Rheumatol; Effectiveness of tofacitinib monotherapy for patients with IgG4-RD or idiopathic retroperitoneal fibrosis; Authors - Xiaoyu Cao
mouse	WB	Int Immunopharmacol; Demethylzeylasteral alleviates inflammation and colitis via dual suppression of NF-κB and STAT3/5 by targeting IKKα/β and JAK2; Authors - Tian Wen
mouse	WB	Adv Sci (Weinh); Electret-Inspired Charge-Injected Hydrogel for Scar-Free Healing of Bacterially Infected Burns Through Bioelectrical Stimulation and Immune Modulation; Authors - Mujie Liu

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