Anticorps Polyclonal de lapin anti-JAKMIP1

• Phare
• Validé par KD/KO

JAKMIP1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13846-1-AP

Synonymes

Gababrbp, JAKMIP1, JAMIP1, Marlin 1, MARLIN1

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Galerie de données de validation

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  WB analysis of mouse spleen using 13846-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13846-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13846-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13846-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13846-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13846-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 13846-1-AP

  IP Result of anti-JAKMIP1 (IP:13846-1-AP, 3ug; Detection:13846-1-AP 1:1000) with Jurkat cells lysate 4000ug.

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  IHC staining of human gliomas using 13846-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13846-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 13846-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug JAKMIP1 antibody (13846-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules HEK-293, cellules Jurkat, cellules Y79, tissu hépatique de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13846-1-AP cible JAKMIP1 dans les applications de WB, IP, IHC, IF, FC,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: JAKMIP1 Protéine recombinante Ag4816
• Nom complet: janus kinase and microtubule interacting protein 1
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC047075
• Symbole du gène: JAKMIP1
• Identification du gène (NCBI): 152789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF	Neuron; JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.; Authors - Jamee M Berg ; KO Validated
human	WB,IHC	Biomed Pharmacother; Overexpression of JAKMIP1 associates with Wnt/beta-catenin pathway activation and promotes cancer cell proliferation in vitro.; Authors - Okai Isaac I