Anticorps Polyclonal de lapin anti-JAML

JAML Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21302-1-PBS

Synonymes

Gm638, Dendritic cell-specific protein CREA7-1, CREA7 4, CREA7 1, AMICA1

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Galerie de données de validation

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  WB analysis using 21302-1-AP (same clone as 21302-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21302-1-AP (JAML antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21302-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 21302-1-AP (same clone as 21302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 21302-1-AP (JAML antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21302-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 21302-1-AP (same clone as 21302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 21302-1-AP (JAML antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21302-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21302-1-PBS cible JAML dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: JAML Protéine recombinante Ag15900
• Nom complet: adhesion molecule, interacts with CXADR antigen 1
• Masse moléculaire calculée: 394 aa, 44 kDa
• Poids moléculaire observé: 44 kDa, 50-70 kDa
• Numéro d’acquisition GenBank: BC100797
• Symbole du gène: JAML
• Identification du gène (NCBI): 120425
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Junctional adhesion molecules (JAM-A, JAM-B, and JAM-C) are endothelial and epithelial adhesion molecules involved in the recruitment of circulating leukocytes to inflammatory sites (PMID: 19064666). JAML (junctional adhesion molecule-like), also known as AMICA1, is related to JAM proteins. It contains 2 extracellular immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail. JAML is expressed on leukocytes and promotes their adhesion to endothelial cells (PMID: 12869515). JAML can regulate neutrophil transepithelial migration via binding interactions with epithelial coxsackie and adenovirus receptor (CAR) (PMID: 15800062). The full-length human JAML protein has a calculated molecular weight of 44 kDa. This antibody raised against 40-275 aa of human JAML protein detects bands with molecular wight of 44 kDa, and 50-70 kDa which probably represent different glycoforms of the protein (PMID: 12869515; 24621992; 15800062).

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