Anticorps Polyclonal de lapin anti-JMJD3

JMJD3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 55354-1-AP

Synonymes

JMJD3, KDM6B, KIAA0346, Lysine-specific demethylase 6B

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Galerie de données de validation

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  WB analysis using 55354-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55354-1-AP (JMJD3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

55354-1-AP cible JMJD3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: jumonji domain containing 3, histone lysine demethylase
• Masse moléculaire calculée: 177 kDa
• Poids moléculaire observé: 177 kDa
• Numéro d’acquisition GenBank: NM_001080424
• Symbole du gène: JMJD3
• Identification du gène (NCBI): 23135
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

JMJD3, also known as KDM6B, is a 1643 amino acid protein, which belongs to the UTX family. JMJD3 is a Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17825402, PubMed:17851529, ). JMJD3 demethylates trimethylated and dimethylated H3 'Lys-27', so it Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529). Histone demethylases are epigenetic actors with a crucial role in cancer by acting as suppressors of tumors or as oncogenes. JMJD3 and UTX (ubiquitously transcribed tetratricopeptide repeat, X chromosome) are transcription activators, being specific H3K27me3 demethylases. JMJD3 is involved in many cellular process such as development, differentiation, senescence and aging by p16, p53 and RB pathways and finally inflammation. Depending on cancer type, JMJD3 expression is increased (prostate and breast cancers, melanoma, gliomas, renal cell carcinoma or decreased (lung, liver, pancreatic, colon and colorectal cancers. This role in carcinogenesis has allowed the development of "epidrugs" to modulate JMJD3 expression (PMID: 29805743 ).

Publications

Species	Application	Title
human	WB	Inflammation; Glycoprotein Nonmetastatic Melanoma Protein B (GPNMB) Ameliorates the Inflammatory Response in Periodontal Disease.; Authors - Rong Song
mouse	WB	Gene; miR-148a-3p regulates adipocyte and osteoblast differentiation by targeting lysine-specific demethylase 6b.; Authors - Lijie Tian
mouse	WB	J Physiol; Histone demethylase UTX is a therapeutic target for diabetic kidney disease.; Authors - Hong Chen
rat	WB	Stem Cells Dev; Inhibition of Ezh2 In Vitro and the Decline of Ezh2 in Developing Midbrain Promote Dopaminergic Neurons Differentiation Through Modifying H3K27me3.; Authors - Feng Hong
human	WB	Oncol Rep; Targeting P16INK4A in uterine serous carcinoma through inhibition of histone demethylation.; Authors - Zhen Xiao