Anticorps Polyclonal de lapin anti-JMJD6

• Phare
• Validé par KD/KO

JMJD6 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16476-1-PBS

Synonymes

Lysyl-hydroxylase JMJD6, KIAA0585, Jumonji domain-containing protein 6, JmjC domain-containing protein 6, EC:1.14.11.-

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Galerie de données de validation

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  WB analysis of mouse liver using 16476-1-AP (same clone as 16476-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16476-1-AP (JMJD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 16476-1-AP (same clone as 16476-1-PBS)

  IP result of anti-JMJD6 (IP:16476-1-AP, 4ug; Detection:16476-1-AP 1:500) with K-562 cells lysate 1200ug. This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16476-1-AP (same clone as 16476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 16476-1-AP (same clone as 16476-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.4 ug JMJD6 Polyclonal antibody (16476-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16476-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16476-1-PBS cible JMJD6 dans les applications de WB, IHC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: JMJD6 Protéine recombinante Ag9590
• Nom complet: jumonji domain containing 6
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC066654
• Symbole du gène: JMJD6
• Identification du gène (NCBI): 23210
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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