Anticorps Polyclonal de lapin anti-JNK

JNK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 51151-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 51151-1-AP (same clone as 51151-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

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  WB analysis using 51151-1-AP (same clone as 51151-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 51151-1-AP (same clone as 51151-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 51151-1-AP (same clone as 51151-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 51151-1-AP (same clone as 51151-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 51151-1-AP (same clone as 51151-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 51151-1-AP (same clone as 51151-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 51151-1-AP (same clone as 51151-1-PBS)

  Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 51151-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51151-1-PBS in a different storage buffer formulation.

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Informations sur le produit

51151-1-PBS cible JNK dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase 8
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 44-48 kDa, 50-55 kDa
• Numéro d’acquisition GenBank: NM_138982
• Symbole du gène: JNK
• Identification du gène (NCBI): 5599
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

• JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

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