Anticorps Monoclonal anti-JNK

• Phare
• Validé par KD/KO

JNK Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A12E1

N° de cat : 66210-1-PBS

Synonymes

MAPK8, 1A12E1, c Jun N terminal kinase 1, EC:2.7.11.24, JNK 1

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Galerie de données de validation

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  WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  WB analysis using 66210-1-Ig (same clone as 66210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66210-1-Ig (same clone as 66210-1-PBS)

  Ethacrynic acid treated HeLa cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66210-1-Ig (same clone as 66210-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 66210-1-Ig (same clone as 66210-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66210-1-Ig (JNK Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66210-1-Ig (same clone as 66210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66210-1-Ig (same clone as 66210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66210-1-Ig (same clone as 66210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66210-1-Ig (JNK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 66210-1-Ig (same clone as 66210-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JNK antibody (66210-1-Ig, Clone: 1A12E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66210-1-Ig (same clone as 66210-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human JNK (66210-1-Ig, Clone:1A12E1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66210-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66210-1-PBS cible JNK dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: JNK Protéine recombinante Ag21426
• Nom complet: mitogen-activated protein kinase 8
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 44-48 kDa, 50-55 kDa
• Numéro d’acquisition GenBank: BC130572
• Symbole du gène: JNK
• Identification du gène (NCBI): 5599
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

• JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

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