Anticorps Monoclonal anti-JTV1

JTV1 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D6B5

N° de cat : 66848-1-PBS

Synonymes

PRO0992, P38, HLD17, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, AIMP2

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Galerie de données de validation

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  WB analysis using 66848-1-Ig (same clone as 66848-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66848-1-Ig (JTV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

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  WB analysis using 66848-1-Ig (same clone as 66848-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66848-1-Ig (JTV1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66848-1-Ig (same clone as 66848-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66848-1-Ig (JTV1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66848-1-Ig (same clone as 66848-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66848-1-Ig (JTV1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66848-1-Ig (same clone as 66848-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug JTV1 Monoclonal antibody (66848-1-Ig, Clone:1D6B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66848-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66848-1-PBS cible JTV1 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: JTV1 Protéine recombinante Ag0675
• Nom complet: JTV1 gene
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 32-36 kDa
• Numéro d’acquisition GenBank: BC002853
• Symbole du gène: JTV1
• Identification du gène (NCBI): 7965
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

JTV1, also named p38, AIMP2, was first knew as a factor associated with macromolecular protein complex, which consists several different aminoacyl-tRNA synthetases. JTV1 is a scaffold protein required for the assembly and stability of the multi-tRNA synthetase complex. JTV1 could act as a mediator of TGF-beta signaling and its functional importance in the control of MYC during lung differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.

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