Anticorps Recombinant de lapin anti-JUNB

JUNB Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

242562G2

N° de cat : 84922-6-RR

Synonymes

242562G2, AP 1, AP-1

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Galerie de données de validation

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  WB analysis using 84922-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84922-6-RR (JUNB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 84922-6-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84922-6-RR (JUNB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84922-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUNB antibody (84922-6-RR, Clone: 242562G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 84922-6-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using JUNB antibody (84922-6-RR, Clone: 242562G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84922-6-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug JUNB Recombinant antibody (84922-6-RR, Clone:242562G2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  ChIP experiment of PC-12 using 84922-6-RR

  Chromatin was prepared from PC-12 cells starved overnight and treated with β-NGF (50 ng/ml) for 2 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of JUNB (84922-6-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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  Affinity and Kinetic Characterization of 84922-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84922-6-RR against Human JUNB were performed. The affinity constant is 0.213 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431
• Résultats positifs en FC (Intra): cellules HeLa,
• Résultats positifs en ChIP-qPCR: β-NGF(starved overnight) (50 ng/ml, 2 h) PC-12 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84922-6-RR cible JUNB dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: JUNB Protéine recombinante Ag0752
• Nom complet: jun B proto-oncogene
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 38 kDa, 42 kDa
• Numéro d’acquisition GenBank: BC004250
• Symbole du gène: JUNB
• Identification du gène (NCBI): 3726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context

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