Anticorps Recombinant de lapin anti-JUND

JUND Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

230457A4

N° de cat : 83134-6-PBS

Synonymes

230457A4, AP 1, jun D proto oncogene, Transcription factor jun D

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Galerie de données de validation

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  WB analysis using 83134-6-RR (same clone as 83134-6-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 83134-6-RR (JUND antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83134-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83134-6-RR (same clone as 83134-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JUND antibody (83134-6-RR, Clone: 230457A4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83134-6-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83134-6-RR (same clone as 83134-6-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug JUND Recombinant antibody (83134-6-RR, Clone:230457A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83134-6-PBS in a different storage buffer formulation.

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  ChIP experiment of HCT 116 using 83134-6-RR (same clone as 83134-6-PBS)

  Chromatin was prepared from HCT 116 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of JUND (83134-6-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83134-6-PBS in a different storage buffer formulation.

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Informations sur le produit

83134-6-PBS cible JUND dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: jun D proto-oncogene
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35-45 kDa
• Numéro d’acquisition GenBank: NM_005354
• Symbole du gène: JUND
• Identification du gène (NCBI): 3727
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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