Anticorps Polyclonal de lapin anti-KALRN

• Phare
• Validé par KD/KO

KALRN Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 19740-1-AP

Synonymes

DUET, duo, HAPIP, Kalirin, kalirin, RhoGEF kinase, KALRN, Protein Duo, TRAD

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Galerie de données de validation

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  WB analysis using 19740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19740-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 19740-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 19740-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19740-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19740-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19740-1-AP (KALRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19740-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19740-1-AP (KALRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19740-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 19740-1-AP (KALRN antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, tissu cérébral humain, tissu de côlon de rat, tissu rénal humain
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

19740-1-AP cible KALRN dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: kalirin, RhoGEF kinase
• Masse moléculaire calculée: 340 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: NM_007064
• Symbole du gène: KALRN
• Identification du gène (NCBI): 8997
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KALRN, also named as DUET, DUO, HAPIP and TRAD, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. KALRN promotes the exchange of GDP by GTP. KALRN activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. KALRN induces lamellipodia independent of its GEF activity. The antibody can recognize isoform1 and isoform2 of KALRN.

Publications

Species	Application	Title
mouse	WB	FEBS Lett; Kalirin mediates Rac1 activation downstream of calcium/calmodulin-dependent protein kinase II to stimulate glucose uptake during muscle contraction.; Authors - Sasa Liu
mouse	WB	Biochem Biophys Res Commun; CaMKII and Kalirin, a Rac1-GEF, regulate Akt phosphorylation involved in contraction-induced glucose uptake in skeletal muscle cells.; Authors - Sasa Liu
human	WB,IF	BMC Womens Health; Metabolism-associated molecular classification of cervical cancer; Authors - Min Zhao ; KD Validated
rat	WB	Mol Cell Neurosci; Role and mechanism of EphB3 in epileptic seizures and epileptogenesis through Kalirin; Authors - Hao Huang