Anticorps Polyclonal de lapin anti-KALRN

• Phare
• Validé par KD/KO

KALRN Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19740-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 19740-1-AP (same clone as 19740-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 19740-1-AP (same clone as 19740-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 19740-1-AP (same clone as 19740-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 19740-1-AP (same clone as 19740-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 19740-1-AP (same clone as 19740-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 19740-1-AP (KALRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19740-1-AP (same clone as 19740-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19740-1-AP (KALRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19740-1-AP (same clone as 19740-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19740-1-AP (KALRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19740-1-AP (same clone as 19740-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 19740-1-AP (KALRN antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19740-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19740-1-PBS cible KALRN dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: kalirin, RhoGEF kinase
• Masse moléculaire calculée: 340 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: NM_007064
• Symbole du gène: KALRN
• Identification du gène (NCBI): 8997
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KALRN, also named as DUET, DUO, HAPIP and TRAD, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. KALRN promotes the exchange of GDP by GTP. KALRN activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. KALRN induces lamellipodia independent of its GEF activity. The antibody can recognize isoform1 and isoform2 of KALRN.

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