Anticorps Polyclonal de lapin anti-KANK1

KANK1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29782-1-PBS

Synonymes

KN motif and ankyrin repeat domain-containing protein 1, Kidney ankyrin repeat-containing protein, KIAA0172, KANK, Ankyrin repeat domain-containing protein 15

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Galerie de données de validation

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  WB analysis using 29782-1-AP (same clone as 29782-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29782-1-AP (KANK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29782-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 29782-1-AP (same clone as 29782-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29782-1-AP (KANK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29782-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 29782-1-AP (same clone as 29782-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29782-1-AP (KANK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29782-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 29782-1-AP (same clone as 29782-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using KANK1 antibody (29782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29782-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29782-1-PBS cible KANK1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KANK1 Protéine recombinante Ag31422
• Nom complet: KN motif and ankyrin repeat domains 1
• Masse moléculaire calculée: 147KD
• Poids moléculaire observé: 130-200 kDa
• Numéro d’acquisition GenBank: NM_015158
• Symbole du gène: KANK1
• Identification du gène (NCBI): 23189
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KANK1 (KN motif and ankyrin repeat domain-containing protein 1) is involved in the control of cytoskeleton formation by regulating actin polymerization, inhibiting actin fiber formation and cell migration (PMID: 25961457). It inhibits RhoA activity, the formation of lamellipodia but not of filopodia (PMID: 25961457, PMID: 5961457).It also inhibits fibronectin-mediated cell spreading and regulates Rac signaling pathways (PMID: 25961457). Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions (PMID: 27410476). KANK1 can be detected 130-200 kDa (PMID: 15823577, PMID: 33253712, PMID: 35805114).

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