Anticorps Monoclonal anti-KAP1

• Phare
• Validé par KD/KO

KAP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B9G12

N° de cat : 66630-1-PBS

Synonymes

TRIM28, 1B9G12, E3 SUMO-protein ligase TRIM28, EC:2.3.2.27, KAP 1

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Galerie de données de validation

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  WB analysis of HeLa using 66630-1-Ig (same clone as 66630-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66630-1-Ig (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

  WB result of KAP1 antibody (66630-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAP1 transfected HepG2 cells. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  WB analysis using 66630-1-Ig (same clone as 66630-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66630-1-Ig (KAP1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66630-1-Ig (same clone as 66630-1-PBS)

  IP result of anti-KAP1 (IP:66630-1-Ig, 5ug; Detection:66630-1-Ig 1:1000) with HeLa cells lysate 4000 ug. This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66630-1-Ig (KAP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66630-1-Ig (same clone as 66630-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (66630-1-Ig, Clone: 1B9G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66630-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66630-1-PBS cible KAP1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KAP1 Protéine recombinante Ag7519
• Nom complet: tripartite motif-containing 28
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC004978
• Symbole du gène: KAP1
• Identification du gène (NCBI): 10155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )

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