Anticorps Polyclonal de lapin anti-KARS

• Phare
• Validé par KD/KO

KARS Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 14951-1-AP

Synonymes

KARS, KARS2, KIAA0070, KRS, Lysine tRNA ligase, LysRS, lysyl tRNA synthetase

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Galerie de données de validation

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  WB analysis using 14951-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14951-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 14951-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14951-1-AP (KARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 14951-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14951-1-AP (KARS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14951-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14951-1-AP (KARS antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 14951-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using KARS antibody (14951-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules Jurkat, tissu de côlon humain
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 2 publications below

Informations sur le produit

14951-1-AP cible KARS dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KARS Protéine recombinante Ag6778
• Nom complet: lysyl-tRNA synthetase
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC004132
• Symbole du gène: KARS
• Identification du gène (NCBI): 3735
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB	Sci Adv; Lysyl-tRNA synthetase produces diadenosine tetraphosphate to curb STING-dependent inflammation.; Authors - J Guerra
zebrafish	WB	Nucleic Acids Res; Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.; Authors - Qinghai Zhang
human	WB	Nucleic Acids Res; Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.; Authors - Haissi Cui
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	Hum Mutat; Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.; Authors - Benedetta Ruzzenente
human	IF	Front Cell Infect Microbiol; Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.; Authors - Tian Luo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kishor (Verified Customer) (01-30-2019)

This antibody working well against rat liver tissue and human cancer cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue

KARS Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:14951-1-AP)