Anticorps Monoclonal anti-KAT2A/GCN5

• Phare
• Validé par KD/KO

KAT2A/GCN5 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

1F6G7

N° de cat : 66575-1-Ig

Synonymes

GCN5, GCN5L2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, K(lysine) acetyltransferase 2A, KAT2A, KAT2A/GNC5, Lysine acetyltransferase 2A, PCAF b, STAF97

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Galerie de données de validation

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  WB analysis using 66575-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66575-1-Ig (KAT2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66575-1-Ig

  WB result of KAT2A/GNC5 antibody (66575-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAT2A/GNC5 transfected MCF-7 cells.

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  WB analysis of HSC-T6 using 66575-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66575-1-Ig (KAT2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66575-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66575-1-Ig (KAT2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HSC-T6, cellules MCF-7, cellules NIH/3T3, cellules SKOV-3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

66575-1-Ig cible KAT2A/GCN5 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KAT2A/GCN5 Protéine recombinante Ag6940
• Nom complet: K(lysine) acetyltransferase 2A
• Masse moléculaire calculée: 94 kDa
• Poids moléculaire observé: 94 kDa
• Numéro d’acquisition GenBank: BC032743
• Symbole du gène: KAT2A
• Identification du gène (NCBI): 2648
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.

Publications

Species	Application	Title
mouse	WB	Brain Behav Immun; Kynurenine pathway is altered in BDNF Val66Met knock-in mice: Effect of physical exercise.; Authors - Alessandro Ieraci
mouse	WB	J Bone Miner Res; Inhibition of ACLY Leads to Suppression of Osteoclast Differentiation and Function Via Regulation of Histone Acetylation.; Authors - Qian Guo
human	WB	Development; Dynamic profiling and functional interpretation of histone Kcr and Kla during neural development.; Authors - Shang-Kun Dai ; KD Validated
human	WB,IF	PLoS One; Psychostimulants and opioids differentially influence the epigenetic modification of histone acetyltransferase and histone deacetylase in astrocytes.; Authors - Mayur Doke
human	WB	BMC Cancer; Histone regulator KAT2A acts as a potential biomarker related to tumor microenvironment and prognosis of diffuse large B cell lymphoma; Authors - Zhuoya Yu ; KD Validated