Anticorps Monoclonal anti-KBTBD2

• Phare
• Validé par KD/KO

KBTBD2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A9F3

N° de cat : 67895-1-PBS

Synonymes

BKLHD1, KBTBD2, KIAA1489

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Galerie de données de validation

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  WB analysis using 67895-1-Ig (same clone as 67895-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67895-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67895-1-Ig (same clone as 67895-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67895-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67895-1-Ig (same clone as 67895-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KBTBD2 antibody (67895-1-Ig, Clone: 2A9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue). This data was developed using the same antibody clone with 67895-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67895-1-PBS cible KBTBD2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: KBTBD2 Protéine recombinante Ag4933
• Nom complet: kelch repeat and BTB (POZ) domain containing 2
• Masse moléculaire calculée: 622 aa, 71 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC047107
• Symbole du gène: KBTBD2
• Identification du gène (NCBI): 25948
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106).

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