Anticorps Polyclonal de lapin anti-KCNA3

• Phare
• Validé par KD/KO

KCNA3 Polyclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 14079-1-AP

Synonymes

Kv1.3, MK3, HUKIII, HPCN3, HLK3

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Galerie de données de validation

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  WB analysis of A549 using 14079-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14079-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14079-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of Rat brain using 14079-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Rat brain tissue using Kv1.3 antibody (14079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, tissu de thymus de souris
• Résultats positifs en IF-P: tissu cérébral de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 5 publications below

Informations sur le produit

14079-1-AP cible KCNA3 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCNA3 Protéine recombinante Ag5204
• Nom complet: potassium voltage-gated channel, shaker-related subfamily, member 3
• Masse moléculaire calculée: 575 aa, 64 kDa
• Poids moléculaire observé: 52 kDa, 75 kDa
• Numéro d’acquisition GenBank: BC035059
• Symbole du gène: KCNA3
• Identification du gène (NCBI): 3738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Kv1.3 ( also known as KCNA3, MK3, HGK5, HLK3, PCN3, HPCN3) contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. It plays an essential role in T cell proliferation and activation. Kv1.3 channels are expressed in in various tissues and cell types including brain, vascular smooth muscle cells and leucocytes. Malfunction of Kv1.3 can alter the immune response, elicit neurotoxic effects or impact on cancer growth(PMID: 24305723; 24133455). 14079-1-AP can detect two isoforms of Kv1.3 other than the canonical channel (60 KDa) : A longer isoform (70 KDa) and a truncated isoform (43 KDa).

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology.; Authors - Xiaolong He ; KD Validated
mouse	WB,IF	Int Immunopharmacol; Clarithromycin attenuates airway epithelial-mesenchymal transition in ovalbumin-induced asthmatic mice through modulation of Kv1.3 channels and PI3K/Akt signaling; Authors - Bingqing Sun
human	WB	FASEB J; The Kv1.3 ion channel acts as a host factor restricting viral entry.; Authors - Yange Lang
rat	WB	Int J Clin Exp Med; Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1.; Authors - Wenjuan Liu
mouse	WB,IF	Cell Death Discov; Therapeutic potential of voltage-dependent potassium channel subtype 1.3 blockade in alleviating macrophage-related renal inflammation and fibrogenesis; Authors - Sha-Sha Li
human,mouse	WB,IF	Transl Res; Kv1.3 expression on CD4 (+) T cells promotes interleukin-17A-associated airway inflammation and airway remodeling in asthma; Authors - Bingqing Sun

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