Anticorps Polyclonal de lapin anti-KCNAB2

KCNAB2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 17890-1-AP

Synonymes

AKR6A5, HKvbeta2, HKvbeta2.1, HKvbeta2.2, K(+) channel subunit beta 2, KCNA2B, KCNAB2, KCNK2, KV BETA 2, TREK, TREK1

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Galerie de données de validation

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  WB analysis of mouse brain using 17890-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17890-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17890-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HepG2
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

17890-1-AP cible KCNAB2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCNAB2 Protéine recombinante Ag12096
• Nom complet: potassium voltage-gated channel, shaker-related subfamily, beta member 2
• Masse moléculaire calculée: 395 aa, 44 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC110351
• Symbole du gène: KCNAB2
• Identification du gène (NCBI): 8514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	IHC	Sci Rep; The long non-coding RNA NEAT1 is responsive to neuronal activity and is associated with hyperexcitability states.; Authors - Guy Barry
human	WB	Brain Sci; Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells.; Authors - Tsung-Ming Hu
human	WB	Cells; The Ion Channel Gene KCNAB2 Is Associated with Poor Prognosis and Loss of Immune Infiltration in Lung Adenocarcinoma; Authors - Yin Lyu
human	WB	Acta Biochim Biophys Sin (Shanghai); Screening of autoantibodies as biomarkers in the serum of renal cancer patients based on human proteome microarray; Authors - Yangyang Sun

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