Anticorps Polyclonal de lapin anti-Kv4.2

• Phare
• Validé par KD/KO

Kv4.2 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 21298-1-AP

Synonymes

KCND2, KIAA1044, Kv4.2, RK5

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Galerie de données de validation

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  WB analysis of A549 using 21298-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21298-1-AP (Kv4.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21298-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21298-1-AP (Kv4.2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 21298-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 21298-1-AP (Kv4.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21298-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21298-1-AP (Kv4.2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21298-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21298-1-AP (Kv4.2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 21298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 21298-1-AP (Kv4.2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 21298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 21298-1-AP (Kv4.2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules DU 145, cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

21298-1-AP cible Kv4.2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Kv4.2 Protéine recombinante Ag15879
• Nom complet: potassium voltage-gated channel, Shal-related subfamily, member 2
• Masse moléculaire calculée: 630 aa, 71 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC110449
• Symbole du gène: KCND2
• Identification du gène (NCBI): 3751
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).

Publications

Species	Application	Title
mouse		Neurobiol Dis; MicroRNA inhibition upregulates hippocampal A-type potassium current and reduces seizure frequency in a mouse model of epilepsy.; Authors - Durgesh Tiwari
mouse	WB	Sci Rep; Mir324 knockout regulates the structure of dendritic spines and impairs hippocampal long-term potentiation; Authors - Emma V Parkins
mouse	WB	eNeuro; Estradiol- and Progesterone-Associated Changes in microRNA-Induced Silencing and Reduced Antiseizure Efficacy of an Antagomir in Female Mice; Authors - Durgesh Tiwari
rat	WB,IHC,IF	JCI Insight; Indoxyl sulfate reduces Ito,f by activating ROS/MAPK and NF-κB signaling pathways.; Authors - Jing Yang
rat	WB	Biochem Biophys Res Commun; Pinocembrin alleviates the susceptibility to atrial fibrillation in isoproterenol-induced rats; Authors - Zhangchi Liu
mouse	WB	Res Sq; Age-dependent regulation of dendritic spine density and protein expression in Mir324 KO mice; Authors - Emma V Parkins