Anticorps Recombinant de lapin anti-Kv4.2

Kv4.2 Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242754F4

N° de cat : 85133-3-PBS

Synonymes

KCND2, 242754F4, KIAA1044, Potassium voltage-gated channel subfamily D member 2, RK5

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Galerie de données de validation

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  WB analysis using 85133-3-RR (same clone as 85133-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 85133-3-RR (same clone as 85133-3-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 85133-3-RR (same clone as 85133-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85133-3-RR (Kv4.2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 85133-3-RR (same clone as 85133-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85133-3-RR (Kv4.2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 85133-3-RR (same clone as 85133-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Kv4.2 antibody (85133-3-RR, Clone: 242754F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01834-2

  Cytometric bead array standard curve of MP01834-2, Kv4.2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85133-4-PBS. Detection antibody: 85133-3-PBS. Standard: Ag15879. Range: 1.563-100 ng/mL.

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  Affinity and Kinetic Characterization of 85133-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85133-3-RR against Human Kv4.2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85133-3-PBS cible Kv4.2 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Kv4.2 Protéine recombinante Ag15879
• Nom complet: potassium voltage-gated channel, Shal-related subfamily, member 2
• Masse moléculaire calculée: 630 aa, 71 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC110449
• Symbole du gène: KCND2
• Identification du gène (NCBI): 3751
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).

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