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Anticorps Polyclonal de lapin anti-KCNJ1

KCNJ1 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20953-1-PBS

Synonymes

Galerie de données de validation

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  • Western Blot (WB) analysis of mouse brain tissue using KCNJ1 Polyclonal antibody (20953-1-AP)

    WB analysis of mouse brain using 20953-1-AP (same clone as 20953-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20953-1-AP (KCNJ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20953-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using KCNJ1 Polyclonal antibody (20953-1-AP)

    WB analysis of mouse brain using 20953-1-AP (same clone as 20953-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20953-1-AP (KCNJ1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20953-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KCNJ1 Polyclonal antibody (20953-1-AP)

    IP experiment of mouse brain using 20953-1-AP (same clone as 20953-1-PBS)

    IP result of anti-KCNJ1 (IP:20953-1-AP, 4ug; Detection:20953-1-AP 1:300) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 20953-1-PBS in a different storage buffer formulation.

Informations sur le produit

20953-1-PBS cible KCNJ1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène KCNJ1 Protéine recombinante Ag15100
Nom complet potassium inwardly-rectifying channel, subfamily J, member 1
Masse moléculaire calculée 391 aa, 45 kDa
Poids moléculaire observé 75-80 kDa
Numéro d’acquisition GenBankBC074752
Symbole du gène KCNJ1
Identification du gène (NCBI) 3758
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KCNJ1, also called ROMK, is an inward-rectifying K+ channel responsible for basal (non-flow stimulated) K+ secretion. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium. This channel is activated by internal ATP and can be blocked by external barium. The calculated molecule weight is 45kDa, 20953-1-AP can detect the band around 75-80 kDa, it has been considered to be a dimer of KCNJ1.(PMID: 17804670; 7929082; 15767585)

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