Anticorps Polyclonal de lapin anti-Kir4.1

Kir4.1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12503-1-AP

Synonymes

BIRK 10, KCNJ10, KCNJ13 PEN, KIR1.2, Kir4.1

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Galerie de données de validation

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  WB analysis using 12503-1-AP

  mouse brain tissues (heated at 100°C, unboided, heated at 37°C) were subjected to SDS PAGE followed by western blot with 12503-1-AP (Kir4.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12503-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12503-1-AP (Kir4.1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 12503-1-AP

  IP Result of anti-Kir4.1 (IP:12503-1-AP, 4ug; Detection:12503-1-AP 1:500) with rat brain tissue lysate 4800ug.

• 

  IHC staining of human gliomas using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 12503-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

12503-1-AP cible Kir4.1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Kir4.1 Protéine recombinante Ag3182
• Nom complet: potassium inwardly-rectifying channel, subfamily J, member 10
• Masse moléculaire calculée: 379 aa, 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC034036
• Symbole du gène: KCNJ10
• Identification du gène (NCBI): 3766
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Neuron; Glioma epileptiform activity and progression are driven by IGSF3-mediated potassium dysregulation; Authors - Rachel Naomi Curry
		Glia; Wnt/β-catenin signaling mediates the seizure-facilitating effect of postischemic reactive astrocytes after pentylenetetrazole-kindling.; Authors - Jialei Yang
mouse	IF	Glia; Elevated GFAP induces astrocyte dysfunction in caudal brain regions: A potential mechanism for hindbrain involved symptoms in type II Alexander disease.; Authors - Heather R Minkel
human	WB	Mult Scler; Low reliability of anti-KIR4.183-120 peptide auto-antibodies in multiple sclerosis patients.; Authors - Mariapaola Marino
mouse,rabbit	WB	Exp Eye Res; A novel model of subretinal edema induced by DL-alpha aminoadipic acid; Authors - Zhan Xie
rat	WB,IF	Am J Transl Res; Yigan Mingmu Decoction inhibits diabetic macular edema through regulating Kir4.1/AQP4 axis: a study based on network pharmacology; Authors - Longrong Huang

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