Anticorps Recombinant de lapin anti-KCNJ18

KCNJ18 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241381A2

N° de cat : 84203-5-PBS

Synonymes

TTPP2, KIR2.6, Inward rectifier potassium channel 18, 241381A2

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Galerie de données de validation

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  WB analysis using 84203-5-RR (same clone as 84203-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84203-5-RR (KCNJ18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84203-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 84203-5-RR (same clone as 84203-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84203-5-RR (KCNJ18 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84203-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 84203-5-RR (same clone as 84203-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84203-5-RR (KCNJ18 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84203-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 84203-5-RR (same clone as 84203-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using KCNJ18 antibody (84203-5-RR, Clone: 241381A2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84203-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84203-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84203-5-RR against Human KCNJ18 were performed. The affinity constant is below 3.06 nM.

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Informations sur le produit

84203-5-PBS cible KCNJ18 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KCNJ18 Protéine recombinante Ag34177
• Nom complet: similar to hkir2.2x
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_001194958
• Symbole du gène: KCNJ18
• Identification du gène (NCBI): 100134444
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mutations in KCNJ18 gene encoding an inwardly rectifying potassium channel (Kir2.6) cause thyrotoxic periodic paralysis (TPP) and sporadic, that is, non-familial cases of HypoPP. Mutant Kir2.6 proteins form a heterotetrameric Kir channel complex with Kir2.1 and thereby reduce cell surface expression of the complex. (PMID: 25882930)

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