Anticorps Polyclonal de lapin anti-KCNK18

KCNK18 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 23712-1-AP

Synonymes

K2p18.1, KCNK18, TRESK, TRESK 2, TRESK2, TRIK

---

Galerie de données de validation

• 

  WB analysis of A431 using 23712-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 23712-1-AP (KCNK18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 23712-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 23712-1-AP (KCNK18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23712-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23712-1-AP (KCNK18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 23712-1-AP

  Immunofluorescent analysis of A375 cells using 23712-1-AP (KCNK18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules A375, cellules MCF-7
• Résultats positifs en IF: cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

23712-1-AP cible KCNK18 dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCNK18 Protéine recombinante Ag20514
• Nom complet: potassium channel, subfamily K, member 18
• Masse moléculaire calculée: 384 aa, 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC152979
• Symbole du gène: KCNK18
• Identification du gène (NCBI): 338567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KCNK18, also named as TRESK and TRIK, is an outward rectifying potassium channel which produces rapidly activating outward rectifier K+ currents. It may function as background potassium channel that sets the resting membrane potential. KCNK18 is inhibited by arachidonic acid and other naturally occurring unsaturated free fatty acids. KCNK18 channel activity is enhanced by volatile anesthetics, such as isoflurane. The gene of KCNK18 maps to chromosome 10q26.11, and encodes a 384-amino acid protein with a predicted unmodified molecular weight of 44 kDa. This antibody can be used to detect endogenous KCNK18, however, the reason for the additional band of 130-140 kDa is still unknown.