Anticorps Polyclonal de lapin anti-KCNMA1

KCNMA1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 22158-1-AP

Synonymes

BK channel, BKCA alpha, BKTM, hSlo, K(VCA)alpha

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Galerie de données de validation

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  WB analysis using 22158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22158-1-AP (KCNMA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 22158-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KCNMA1 antibody (22158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 22158-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KCNMA1 antibody (22158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: mouse brain membrane protein, rat brain membrane protein
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22158-1-AP cible KCNMA1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCNMA1 Protéine recombinante Ag17802
• Nom complet: potassium large conductance calcium-activated channel, subfamily M, alpha member 1
• Masse moléculaire calculée: 1236 aa, 138 kDa
• Poids moléculaire observé: ~130 kDa
• Numéro d’acquisition GenBank: BC137115
• Symbole du gène: KCNMA1
• Identification du gène (NCBI): 3778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calcium-activated potassium channel subunit alpha-1 also known as large conductance calcium-activated potassium channel, subfamily M, alpha member 1 (KCa1.1), or BK channel alpha subunit, is a voltage-gated potassium channel encoded by the KCNMA1 gene and characterized by their large conductance of potassium ions (K+) through cell membranes. KCNMA1 is widely distributed in human cell membranes, especially in muscle cells and neurons. They regulate the excitability of cells through sensitivity to calcium ions (Ca²⁺) and voltage, thereby affecting key physiological processes such as muscle contraction and nerve signal transduction.

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