Anticorps Polyclonal de lapin anti-KCNN4

• Phare
• Validé par KD/KO

KCNN4 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 23271-1-AP

Synonymes

KCa3.1, KCNN4, SK4

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Galerie de données de validation

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  WB analysis of HEK-293 using 23271-1-AP

  WB result of KCNN4 antibody (23271-1-AP, 1:4000) with si-control and si-KCNN4 transfected HEK293 cells.

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  WB analysis using 23271-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23271-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 23271-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using KCNN4 antibody (23271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 23271-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 23271-1-AP (KCNN4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HepG2
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

23271-1-AP cible KCNN4 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCNN4 Protéine recombinante Ag19774
• Nom complet: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
• Masse moléculaire calculée: 427 aa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC015337
• Symbole du gène: KCNN4
• Identification du gène (NCBI): 3783
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The intermediate conductance calcium-activated potassium channel KCNN4, also known as KCa3.1, IK1, or SK4, is a member of the potassium channel KCNN family. KCNN4 is expressed in T cells, mast cells, macrophages, erythrocytes, vascular smooth muscle cells, airway smooth muscle cells, intestinal and airway epithelial cells (PMID: 19681865; 21493782). Functionally, KCNN4 plays a key role in calcium-dependent cell functions, such as proliferation, activation, and migration, in a broad range of cell types (PMID: 21493782).

Publications

Species	Application	Title
human	IF	Cancer Cell Int; KCNN4 may weaken anti-tumor immune response via raising Tregs and diminishing resting mast cells in clear cell renal cell carcinoma.; Authors - Yankang Cui
human	WB,IHC,FC	Cancer Biomark; KCNN4 promotes the progression of lung adenocarcinoma by activating the AKT and ERK signaling pathways; Authors - Ping Xu ; KD Validated
human	WB	Front Physiol; New KCNN4 Variants Associated With Anemia: Stomatocytosis Without Erythrocyte Dehydration; Authors - B. Allegrini
human	IHC	Biochem Biophys Res Commun; Integrated expression profiling of potassium channels identifys KCNN4 as a prognostic biomarker of pancreatic cancer.; Authors - Shuheng Jiang
mouse	WB	Gels; Comprehensive Optimization of Western Blotting; Authors - Dishiwen Liu
human	WB	Int J Mol Sci; Downregulation of IL-8 and IL-10 by the Activation of Ca2+-Activated K+ Channel KCa3.1 in THP-1-Derived M2 Macrophages; Authors - Susumu Ohya