Anticorps Polyclonal de lapin anti-KCTD15

• Phare
• Validé par KD/KO

KCTD15 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 20128-1-AP

Synonymes

BTB/POZ domain-containing protein KCTD15, Potassium channel tetramerization domain-containing protein 15

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Galerie de données de validation

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  WB analysis using 20128-1-AP

  Mouse brain tissues were subjected to SDS PAGE followed by western blot with 20128-1-AP (KCTD15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20128-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20128-1-AP (KCTD15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20128-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 20128-1-AP (KCTD15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 20128-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20128-1-AP (KCTD15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of C6 using 20128-1-AP

  IP result of anti-KCTD15 (IP:20128-1-AP, 4ug; Detection:20128-1-AP 1:500) with C6 cells lysate 1960 ug.

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  IHC staining of mouse spleen using 20128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20128-1-AP (KCTD15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 20128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20128-1-AP (KCTD15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules C6, cellules HEK-293, tissu pulmonaire de souris
• Résultats positifs en IP: cellules C6,
• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

20128-1-AP cible KCTD15 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCTD15 Protéine recombinante Ag13962
• Nom complet: potassium channel tetramerisation domain containing 15
• Masse moléculaire calculée: 283 aa, 32 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC001185
• Symbole du gène: KCTD15
• Identification du gène (NCBI): 79047
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KCTD15 is a member of the KCTD family of proteins, which are involved in various biological processes. KCTD15 plays a role in embryonic development by regulating the neural crest domain. It inhibits neural crest induction by repressing the Wnt/β-catenin signaling pathway. KCTD15 is involved in the NF-κB signaling pathway, which is crucial for immune responses and inflammation. Its deregulation can influence the activation of the IKK-β enzyme complex, triggering the NF-κB pathway. KCTD15's expression levels in peripheral blood cells and leukemia cells suggest its potential as a biomarker for diagnosing and monitoring acute leukemias. Its distinct expression profile in different types of white blood cells highlights its importance in both physiological and pathological processes.

Publications

Species	Application	Title
human	WB	Oncogenesis; KCTD15 inhibits the Hedgehog pathway in Medulloblastoma cells by increasing protein levels of the oncosuppressor KCASH2.; Authors - Eleonora Spiombi ; KD Validated
		Proc Natl Acad Sci U S A; KCTD1 regulation of Adenylyl cyclase type 5 adjusts striatal cAMP signaling; Authors - Yini Liao

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