Anticorps Polyclonal de lapin anti-KCTD2/5/17

• Phare
• Validé par KD/KO

KCTD2/5/17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 15553-1-AP

Synonymes

KCTD5, KCTD2, KCTD17, BTB/POZ domain-containing protein KCTD5

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Galerie de données de validation

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  WB analysis of HeLa using 15553-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15553-1-AP (KCTD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15553-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15553-1-AP (KCTD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15553-1-AP

  IP result of anti-KCTD2/5/17 (IP:15553-1-AP, 4ug; Detection:15553-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human ovary tumor using 15553-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15553-1-AP (KCTD5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 15553-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15553-1-AP (KCTD5 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 15553-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KCTD2/5/17 antibody (15553-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IP: See 1 publications below

Informations sur le produit

15553-1-AP cible KCTD2/5/17 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCTD2/5/17 Protéine recombinante Ag7915
• Nom complet: potassium channel tetramerisation domain containing 5
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC007314
• Symbole du gène: KCTD5
• Identification du gène (NCBI): 54442
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KCTD5 belongs to the human potassium (K+) channel tetramerization domain (KCTD) family of proteins which share sequence similarity with the cytoplasmic domain of voltage-gated K+ channels (Kv channels). The KCTD proteins have relatively conserved N-terminal domains and variable C-termini (PMID: 24268103). KCTD5 interacts with cullin3 and has been proposed to function as substrate adapter for cullin3 based ubiquitin E3 ligases (PMID: 26188516). This polyclonal antibody raised against full-length recombinant protein of human KCTD5 (26 kDa) can cross-react with other KCTD proteins, including KCTD2 (29 kDa), KCTD17 (33-36 kDa).

Publications

Species	Application	Title
human	WB	Sci Adv; Structural basis for the ubiquitination of G protein βγ subunits by KCTD5/Cullin3 E3 ligase; Authors - Wentong Jiang
human	WB	Nat Commun; Knowledge graph-based recommendation framework identifies drivers of resistance in EGFR mutant non-small cell lung cancer.; Authors - Anna Gogleva ; KO Validated
human	WB	Diabetes; HOXC10 Suppresses Browning to Maintain White Adipocyte Identity.; Authors - H Y Angeline Tan
human	WB	Elife; Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC.; Authors - Hao Zeng
rat	WB	PLoS Genet; Conserved properties of Drosophila Insomniac link sleep regulation and synaptic function.; Authors - Qiuling Li
human	WB	Commun Biol; Genome-wide CRISPR screens identify the YAP/TEAD axis as a driver of persister cells in EGFR mutant lung cancer; Authors - Matthias Pfeifer

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