Anticorps Recombinant de lapin anti-KDM3A/JMJD1A

KDM3A/JMJD1A Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240532G11

N° de cat : 83571-2-PBS

Synonymes

KDM3A, KDM3A,JMJD1A, [histone H3]-dimethyl-L-lysine(9) demethylase 3A, 240532G11, EC:1.14.11.65

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Galerie de données de validation

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  WB analysis using 83571-2-RR (same clone as 83571-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83571-2-RR (KDM3A,JMJD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83571-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83571-2-RR (same clone as 83571-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83571-2-RR (KDM3A,JMJD1A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83571-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83571-2-RR (same clone as 83571-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KDM3A,JMJD1A antibody (83571-2-RR, Clone: 240532G11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83571-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 83571-2-RR (same clone as 83571-2-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug KDM3A,JMJD1A Recombinant antibody (83571-2-RR, Clone:240532G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83571-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83571-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83571-2-RR against Human KDM3A,JMJD1A were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83571-2-PBS cible KDM3A/JMJD1A dans les applications de WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KDM3A/JMJD1A Protéine recombinante Ag28687
• Nom complet: lysine (K)-specific demethylase 3A
• Masse moléculaire calculée: 150 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC038297
• Symbole du gène: KDM3A/JMJD1A
• Identification du gène (NCBI): 55818
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC.

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