Anticorps Recombinant de lapin anti-KDM3A/JMJD1A

KDM3A/JMJD1A Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240532E2

N° de cat : 83571-6-RR

Synonymes

KDM3A, KDM3A,JMJD1A, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A

---

Galerie de données de validation

• 

  IF Staining of HepG2 using 83571-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KDM3A,JMJD1A antibody (83571-6-RR, Clone: 240532E2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  FC experiment of HEK-293 using 83571-6-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug KDM3A,JMJD1A Recombinant antibody (83571-6-RR, Clone:240532E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83571-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83571-6-RR against Human KDM3A,JMJD1A were performed. The affinity constant is 6.71 nM.

---

Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HEK-293

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83571-6-RR cible KDM3A/JMJD1A dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KDM3A/JMJD1A Protéine recombinante Ag28687
• Nom complet: lysine (K)-specific demethylase 3A
• Masse moléculaire calculée: 150 kDa
• Numéro d’acquisition GenBank: BC038297
• Symbole du gène: KDM3A/JMJD1A
• Identification du gène (NCBI): 55818
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC.

{{ptg:RelatedPrimaryAntibodies}}