Anticorps Recombinant de lapin anti-VEGFR2/CD309

VEGFR2/CD309 Recombinant Antibody for WB, IHC, FC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230238D7

N° de cat : 83049-4-PBS

Synonymes

KDR, VEGFR2, VEGFR2/KDR, 230238D7, CD309

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Galerie de données de validation

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  WB analysis of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

  human placenta tissue was subjected to SDS PAGE followed by western blot with 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HUVEC using 83049-4-RR (same clone as 83049-4-PBS)

  1x10^6 HUVEC cells were surface stained with 0.25 ug VEGFR2/CD309 Recombinant antibody (83049-4-RR, Clone:230238D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83049-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83049-4-RR against Human KDR were performed. The affinity constant is 4.71 nM.

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Informations sur le produit

83049-4-PBS cible VEGFR2/CD309 dans les applications de WB, IHC, FC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VEGFR2/CD309 Protéine recombinante Eg0218
• Nom complet: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Masse moléculaire calculée: 152 kDa
• Poids moléculaire observé: 150 kDa, 230 kDa
• Numéro d’acquisition GenBank: NM_002253
• Symbole du gène: KDR
• Identification du gène (NCBI): 3791
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

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