Anticorps Recombinant de lapin anti-KHSRP

KHSRP Recombinant Antibody for FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

230189C12

N° de cat : 82931-2-RR

Synonymes

230189C12, Far upstream element-binding protein 2, FBP2, FUBP2, FUSE-binding protein 2

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Galerie de données de validation

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  IP experiment of HeLa using 82931-2-RR

  IP result of anti-KHSRP (IP:82931-2-RR, 4ug; Detection:82931-2-RR 1:2500) with HeLa cells lysate 1050 ug.

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  FC experiment of U-251 using 82931-2-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human KHSRP (82931-2-RR, Clone:230189C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 82931-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82931-2-RR against Human KHSRP were performed. The affinity constant is 1.42 nM.

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Applications testées

• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en FC (Intra): cellules U-251,

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82931-2-RR cible KHSRP dans les applications de FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: KHSRP Protéine recombinante Ag33890
• Nom complet: KH-type splicing regulatory protein
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: NM_003685
• Symbole du gène: KHSRP
• Identification du gène (NCBI): 8570
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

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