Anticorps Polyclonal de lapin anti-Ki-67

• Phare
• Validé par KD/KO

Ki-67 Polyclonal Antibody for IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (5)

Applications

IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1327)

Avis (7)

N° de cat : 27309-1-AP

Synonymes

KI67, MKI67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Ki 67

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Galerie de données de validation

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  IHC staining of human tonsillitis using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27309-1-AP (Ki-67 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27309-1-AP (Ki-67 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of K-562 using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded k562 slide using 27309-1-AP (KI67 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27309-1-AP (KI67 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27309-1-AP (KI67 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27309-1-AP (KI67 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27309-1-AP (KI67 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Insulinoma using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Insulinoma using 27309-1-AP

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 27309-1-AP (KI67 antibody) at dilution of 1:16000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 27309-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Ki-67 antibody (27309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27309-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KI67 antibody (27309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 27309-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27309-1-AP (KI67 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red).

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  IF Staining of HEK-293 using 27309-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using KI67 antibody (27309-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 27309-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KI67 antibody (27309-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 27309-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human KI67 (27309-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, cellules K-562, tissu de cancer de la peau humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu de gliome humain, tissu de lymphome humain, tissu d'insulinome; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du poumon humain,
• Résultats positifs en IF/ICC: cellules HeLa, cellules HEK-293
• Résultats positifs en FC (Intra): cellules Jurkat

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• IHC: See 998 publications below
• IF: See 253 publications below

Informations sur le produit

27309-1-AP cible Ki-67 dans les applications de IHC, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Chèvre, Humain, poisson-zèbre, porc, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ki-67 Protéine recombinante Ag26266
• Nom complet: antigen identified by monoclonal antibody Ki-67
• Masse moléculaire calculée: 359 kDa
• Numéro d’acquisition GenBank: NM_002417
• Symbole du gène: KI67
• Identification du gène (NCBI): 4288
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Publications

Species	Application	Title
	IHC	Signal Transduct Target Ther; FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth; Authors - Wenxia Wei
	IHC	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
	IHC	Mol Cancer; lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2; Authors - Jin Wang
human	IHC	Cancer Discov; Stress Granules determine the Development of Obesity-associated Pancreatic Cancer.; Authors - Guillaume Fonteneau
	IF	Adv Mater; Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration; Authors - Zhuo Li
	IHC	Protein Cell; NDFIP1 limits cellular TAZ accumulation via exosomal sorting to inhibit NSCLC proliferation; Authors - Yirui Cheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-12-2025)

Worked well in Western, also detected a non specific band at 70 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: MSCs

FH

Celine (Verified Customer) (07-09-2025)

Works well in immunoflurescence with fixed cells

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500 O/N
• Cell Tissue Type: glioblastoma cell lines

Ki-67 Antibody Immunofluorescence validation (1:500 O/N dilution) in glioblastoma cell lines (Cat no:27309-1-AP)

FH

Guo-rong (Verified Customer) (08-30-2022)

Clear staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: ARPE-19

Ki-67 Antibody Immunofluorescence validation (1:200 dilution) in ARPE-19 (Cat no:27309-1-AP)

FH

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: ARPE-19

FH

Ana (Verified Customer) (02-15-2022)

Both, 1:50 and 1:100 dilutions worked well

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50, 1:100
• Cell Tissue Type: ARPE-19

FH

kes (Verified Customer) (01-17-2022)

Works at 1:200 dilution for IHC

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: murine tissues

FH

Yan (Verified Customer) (02-18-2020)

1:100 work for cell.

• Applications: Immunofluorescence,

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